Lumière sur les cellules tumorales

Comment un neurochirurgien peut-il être sûr qu'il a bien enlevé toute la tumeur cérébrale de son patient, tout en préservant les tissus sains, lorsqu'il opère ? Pour l'y aider, des chercheurs et médecins collaborent pour mettre au point une caméra capable de distinguer avec fiabilité pendant l'opération, les cellules saines des cellules malades. Pour cela, elle va analyser la fluorescence naturellement émise par les tissus, riche en informations, et comparer le résultat à une banque de données, avant de donner son verdict final : tissu sain ou tissu tumoral. Pour constituer cette base de données, les chercheurs viennent, entre autres, au synchrotron SOLEIL, sur la ligne DISCO, pour établir les caractéristiques des différents types de cellules dans l'ultraviolet.

Premier prix de « Basic science essay »

L'étude dirigée par Darine Abi Haidar au sein du laboratoire IMNC intitulée : « Multimodal optical analysis of urothelial carcinoma and comparison with histopathology » a reçu le Premier prix de « Basic science essay » lors du concours annuel organisé par la société internationale d’endourologie « Endourological society » et designé par le comité scientifique du journal « Journal of Endourology ».  Les résultats préliminaires ont été présentés en séance plénière lors du « Worls Congress of Endourology  2017 » qui a eu lieu à Vancouver au mois de septembre.

Multiphoton Microscopy in the Biomedical Sciences Student Poster Session Awards

Fanny Poulon, doctorante au sein de l'équipe IBIV, vient de remporter le Multiphoton Microscopy in the Biomedical Sciences Student Poster Session Award pour son travail autour de la mise en place d'une banque de données tissulaires pour la discrimination des berges de tumeurs cérébrales.

Son poster s'intitule "Multimodal optical imaging database from tumour brain human tissue: endogenous fluorescence from glioma, metastasis and control tissues".


Publications récentes

Optical properties, spectral, and lifetime measurements of central nervous system tumors in humans

F. Poulon, H. Mehidine, M. Juchaux, P. Varlet, B. Devaux, J. Pallud and D. Abi Haidar

A key challenge of central nervous system tumor surgery is to discriminate between brain regions infiltrated by tumor cells and surrounding healthy tissue. Although monitoring of autofluorescence could potentially be an efficient way to provide reliable information for these regions, we found little information on this subject, and thus we conducted studies of brain tissue optical properties. This particular study focuses on the different optical quantitative responses of human central nervous system tumors and their corresponding controls.

Experimental assessment of the safety and potential efficacy of high irradiance photostimulation of brain tissues

S. Senova, I. Scisniak, C.-C Chiang, I. Doignon, S. Palfi, A. Chaillet, C. Martin and F. Pain

Optogenetics is widely used in fundamental neuroscience. Its potential clinical translation for brain neuromodulation requires a careful assessment of the safety and efficacy of repeated, sustained optical stimulation of large volumes of brain tissues. This study was performed in rats and not in non-human primates for ethical reasons. We studied the spatial distribution of light, potential damage, and non-physiological effects in vivo, in anesthetized rat brains, on large brain volumes, following repeated high irradiance

Endogenous fluorescence analysis: preliminary study revealing the potential of this non-invasive method to study mummified samples

M. Zanello, J. Pallud, S. Jacqueline, A. Augias, P. Varlet, B. Devaux, O. Nielsen, D. Abi Haidar and P. Charlier

Autofluorescence analysis allows new insights on human tissue without any dye in a non-invasive way and therefore seems well suited to study historical samples. An optical set-up recorded emitted autofluorescence in 1/spectral and 2/lifetime domains from different samples’ regions of interest. The studied samples were: a mummified right hand; bog body (Tollund Man) feet, and a Caucasian male hand (control sample). Spectral analysis revealed that mummified hand exhibited broad autofluorescence spectra whereas Tollund Man feet exhibited a weak single peak with

Simulation of nanoparticle-mediated near-infrared thermal therapy using GATE

Vesna Cuplov, Frédéric Pain and Sébastien Jan

Application of nanotechnology for biomedicine in cancer therapy allows for direct delivery of anticancer agents to tumors. An example of such therapies is the nanoparticle-mediated near-infrared hyperthermia treatment. In order to investigate the influence of nanoparticle properties on the spatial distribution of heat in the tumor and healthy tissues, accurate simulations are required. The Geant4 Application for Emission Tomography (GATE) open-source simulation platform, based on the Geant4 toolkit, is widely used by the research community involved in

Multimodal optical analysis discriminates freshly extracted human sample of gliomas, metastases and meningiomas from their appropriate controls

M. Zanello, F. Poulon, J. Pallud, P. Varlet, H. Hamzeh, G. Abi Lahoud. F. Andreiuolo, A. Ibrahim, M. Pages, F. Chretien, F. Di Rocco, E. Dezamis, F. Nataf, B. Turak, B. Devaux and Darine Abi Haidar

Delineating tumor margins as accurately as possible is of primordial importance in surgical oncology: extent of resection is associated with survival but respect of healthy surrounding tissue is necessary for preserved quality of life. The real-time analysis of the endogeneous fluorescence signal of brain tissues is a promising tool for defining margins of brain tumors. The present study aims to demonstrate the feasibility of multimodal optical analysis to discriminate fresh samples of gliomas, metastases and meningiomas from their appropriate controls.

Design optimization and performances of an intraoperative positron imaging probe for radioguided cancer surgery

S. Spadola, M.-A. Verdier, L. Pinot, C. Esnault, N. Dinu, Y. Charon, M.-A. Duval and L. Ménard

Extent and accuracy of surgical resection is a crucial step in operable tumor therapy. Emergence of promising specific tumor-seeking agents labeled with positron emitters is giving rise to a renewed interest for radioguided surgery using beta probes. Beta detection, due to the particle short range, allows a more sensitive and accurate tumor localization compared to gamma radiotracers.

Optical properties of mice skull bone in the 455-705 nm range

Soleimanzad H, Gurden H, and Pain F

The activity of cell populations and networks is commonly recorded in vivo in rodents  with wide field optical imaging techniques such as intrinsic optical imaging, fluorescence imaging or laser speckle imaging. These techniques were recently adapted to unrestrained mice carrying transcranial windows. Furthermore, optogenetics studies would benefit from optical stimulation through the skull without implanting an optical fiber, especially for longitudinal studies.

Spectral and fluorescence lifetime endoscopic system using a double-clad photonic crystal fiber

A. Ibrahim, F. Poulon, F. Melouki, M. Zanello, P. Varlet, R. Habert, B. Devaux, A. Kudlinski, and D. Abi Haidar

We present a customized small-core double-clad photonic crystal fiber for spectral and fluorescence lifetime measurements of human samples. In this Letter, the new fiber has been characterized on different fluorophores and samples of human brain tumor; a comparison to a bi-fiber homemade system and a commercial fiber probe was made.

Voir l'article en ligne : ol-41-22-5214

Characterization of fiber ultrashort pulse delivery for nonlinear endomicroscopy

A. Ibrahim, F. Poulon, R. Habert, C. Lefort, A. Kudlinski, and D. Abi Haidar

In this work, we present a detailed characterization of a small-core double-clad photonic crystal fiber, dedicated and approved for in vivo nonlinear imaging endomicroscopy. A numerical and experimental study has been performed to characterize the excitation and collection efficiencies through a 5 m-long optical fiber, including the pulse duration and spectral shape. This was first done without any distal optics, and then the performances of the system were studied by using two kinds of GRIN lenses at the fiber output.
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