M. Zanello, F. Poulon, J. Pallud, P. Varlet, H. Hamzeh, G. Abi Lahoud. F. Andreiuolo, A. Ibrahim, M. Pages, F. Chretien, F. Di Rocco, E. Dezamis, F. Nataf, B. Turak, B. Devaux and Darine Abi Haidar
Delineating tumor margins as accurately as possible is of primordial
importance in surgical oncology: extent of resection is associated with
survival but respect of healthy surrounding tissue is necessary for
preserved quality of life. The real-time analysis of the endogeneous
fluorescence signal of brain tissues is a promising tool for defining
margins of brain tumors. The present study aims to demonstrate the
feasibility of multimodal optical analysis to discriminate fresh samples
of gliomas, metastases and meningiomas from their appropriate controls.
Tumor samples were studied on an optical fibered endoscope using
spectral and fluorescence lifetime analysis and then on a multimodal
set-up for acquiring spectral, one and two-photon fluorescence images,
second harmonic generation signals and two-photon fluorescence lifetime
datasets. The obtained data allowed us to differentiate healthy samples
from tumor samples. These results confirmed the possible clinical
relevance of this real-time multimodal optical analysis. This technique
can be easily applied to neurosurgical procedures for a better
delineation of surgical margins.
Voir l'article en ligne : srep41724
